Nuclease tolerant FRET probe based on DNA-quantum dot conjugation.

نویسندگان

  • Daisuke Onoshima
  • Noritada Kaji
  • Manabu Tokeshi
  • Yoshinobu Baba
چکیده

We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).

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عنوان ژورنال:
  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

دوره 24 2  شماره 

صفحات  -

تاریخ انتشار 2008